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Characterisation of physiological and immunological differences between Pacific oysters (Crassostrea gigas) genetically selected for high or low survival to summer mortalities and fed different rations under controlled conditions ArchiMer
Delaporte, Maryse; Soudant, P; Lambert, C; Jegaden, M; Moal, Jeanne; Pouvreau, Stephane; Degremont, Lionel; Boudry, Pierre; Samain, Jean-francois.
Within the framework of a national scientific program named "MORtalites ESTivales de l'huitre creuse Crassostrea gigas" (MOREST), a family-based experiment was developed to study the genetic basis of resistance to summer mortality in the Pacific oyster, Crassostrea gigas. As part of the MOREST project, the second generation of three resistant families and two susceptible families were chosen and pooled into two respective groups: "R" and "S". These two groups of oysters were conditioned for 6 months on two food levels (4% and 12% of oyster soft-tissue dry weight in algal dry weight per day) with a temperature gradient that mimicked the Marennes-Oleron natural cycle during the oyster reproductive period. Oyster mortality remained low for the first two...
Tipo: Text Palavras-chave: Summer mortality; Reproduction; Reactive oxygen species ROS; Hemocyte parameters; Genetic selection; Crassostrea gigas.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-3578.pdf
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Hemocyte characteristics in families of oysters, Crassostrea gigas, selected for differential survival during summer and reared in three sites ArchiMer
Lambert, Christophe; Soudant, Phillippe; Degremont, Lionel; Delaporte, Maryse; Moal, Jeanne; Boudry, Pierre; Jean, Frédéric; Huvet, Arnaud; Samain, Jean-francois.
High variability among individuals is often encountered when hemocyte characteristics are measured in bivalves. Such variability is suspected to result partly from genetic factors. In this study, hemocyte characteristics of six families of Crassostrea gigas were compared by flow cytometry at one sampling date in October 2001. These families were obtained from a nested, half-sibling cross design, and reared from July to October 2001 at three sites distributed along the French Atlantic coast from north to south: Baie des Veys (Normandy), Riviere d'Auray (Brittany) and Ronce (Marennes-oleron Basin, Poitou Charentes). Among the 15 measured hemocyte characteristics, production of reactive oxygen species (ROS) of untreated hemocytes (maintained in filtered...
Tipo: Text Palavras-chave: Genetic; Flow cytometry; Reactive oxygen species ROS; Adhesion; Hemocyte; Crassostrea gigas; Summer mortality.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-3054.pdf
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